TY - JOUR
T1 - Biophysical evidence of lipid and carbohydrate binding activities of shrimp high density lipoprotein/β glucan binding protein
AU - Vargas-Requena, Claudia L.
AU - Hernández-Santoyo, Alejandra
AU - Yepiz-Plascencia, Gloria
AU - Vargas-Albores, Francisco
AU - Higuera-Ciapara, Inocencio
AU - Rodríguez-Romero, Adela
AU - Soulages, José L.
AU - Sotelo-Mundo, Rogerio R.
PY - 2002/7/30
Y1 - 2002/7/30
N2 - Crustacean High Density Lipoprotein/β-Glucan Binding Protein (HDL/BGBP) has been studied due to its role in nutrition and immune response via activation of the defense cells (hemocytes) upon binding 1,3-D-β-glucan carbohydrates. In this study, HDL/BGBP was found to be composed mainly of beta sheets, as determined by circular dichroism. Lipoprotein aggregation resulted when HDL/BGBP interacted with phospolipid vesicles, laminaribiose (1,3-β-glucan disaccharide) or heparin. HDL/BGBP has similar dissociation constants for laminaribiose (Kd=22 mM) or heparin (Kd=46 mM) as determined by 90° light scattering.
AB - Crustacean High Density Lipoprotein/β-Glucan Binding Protein (HDL/BGBP) has been studied due to its role in nutrition and immune response via activation of the defense cells (hemocytes) upon binding 1,3-D-β-glucan carbohydrates. In this study, HDL/BGBP was found to be composed mainly of beta sheets, as determined by circular dichroism. Lipoprotein aggregation resulted when HDL/BGBP interacted with phospolipid vesicles, laminaribiose (1,3-β-glucan disaccharide) or heparin. HDL/BGBP has similar dissociation constants for laminaribiose (Kd=22 mM) or heparin (Kd=46 mM) as determined by 90° light scattering.
UR - http://www.scopus.com/inward/record.url?scp=0036069413&partnerID=8YFLogxK
U2 - 10.2174/0929866023408706
DO - 10.2174/0929866023408706
M3 - Artículo
C2 - 12144511
AN - SCOPUS:0036069413
SN - 0929-8665
VL - 9
SP - 337
EP - 344
JO - Protein and Peptide Letters
JF - Protein and Peptide Letters
IS - 4
ER -