Cellular viability effects of fatty acid amide hydrolase inhibition on cerebellar neurons

Kathia Lueneberg; Guadalupe Domínguez; Oscar Arias-Carrián; Marcela Palomero-Rivero; Diana Millán-Aldaco; Julio Morán; René Drucker-Colín; Eric Murillo-Rodríguez

doi:10.1186/1755-7682-4-28

Cellular viability effects of fatty acid amide hydrolase inhibition on cerebellar neurons

Kathia Lueneberg, Guadalupe Domínguez, Oscar Arias-Carrián, Marcela Palomero-Rivero, Diana Millán-Aldaco, Julio Morán, René Drucker-Colín, Eric Murillo-Rodríguez

Anahuac Mayab University

Research output: Contribution to journal › Review article › peer-review

6 Scopus citations

Abstract

The endocannabinoid anandamide (ANA) participates in the control of cell death inducing the formation of apoptotic bodies and DNA fragmentation. The aim of this study was to evaluate whether the ANA degrading enzyme, the fatty acid amide hydrolase (FAAH), would induce cellular death. Experiments were performed in cerebellar granule neurons cultured with the FAAH inhibitor, URB597 (25, 50 or 100 nM) as well as endogenous lipids such as oleoylethanolamide (OEA) or palmitoylethanolamide (PEA) and cellular viability was determined by MTT test. Neurons cultured with URB597 (25, 50 or 100 nM) displayed a decrease in cellular viability. In addition, if cultured with OEA (25 nM) or PEA (100 nM), cellular death was found. These results further suggest that URB597, OEA or PEA promote cellular death.

Original language	English
Article number	28
Journal	International Archives of Medicine
Volume	4
Issue number	1
DOIs	https://doi.org/10.1186/1755-7682-4-28
State	Published - 23 Aug 2011

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title = "Cellular viability effects of fatty acid amide hydrolase inhibition on cerebellar neurons",

abstract = "The endocannabinoid anandamide (ANA) participates in the control of cell death inducing the formation of apoptotic bodies and DNA fragmentation. The aim of this study was to evaluate whether the ANA degrading enzyme, the fatty acid amide hydrolase (FAAH), would induce cellular death. Experiments were performed in cerebellar granule neurons cultured with the FAAH inhibitor, URB597 (25, 50 or 100 nM) as well as endogenous lipids such as oleoylethanolamide (OEA) or palmitoylethanolamide (PEA) and cellular viability was determined by MTT test. Neurons cultured with URB597 (25, 50 or 100 nM) displayed a decrease in cellular viability. In addition, if cultured with OEA (25 nM) or PEA (100 nM), cellular death was found. These results further suggest that URB597, OEA or PEA promote cellular death.",

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T1 - Cellular viability effects of fatty acid amide hydrolase inhibition on cerebellar neurons

AU - Lueneberg, Kathia

AU - Domínguez, Guadalupe

AU - Arias-Carrián, Oscar

AU - Palomero-Rivero, Marcela

AU - Millán-Aldaco, Diana

AU - Morán, Julio

AU - Drucker-Colín, René

AU - Murillo-Rodríguez, Eric

PY - 2011/8/23

Y1 - 2011/8/23

N2 - The endocannabinoid anandamide (ANA) participates in the control of cell death inducing the formation of apoptotic bodies and DNA fragmentation. The aim of this study was to evaluate whether the ANA degrading enzyme, the fatty acid amide hydrolase (FAAH), would induce cellular death. Experiments were performed in cerebellar granule neurons cultured with the FAAH inhibitor, URB597 (25, 50 or 100 nM) as well as endogenous lipids such as oleoylethanolamide (OEA) or palmitoylethanolamide (PEA) and cellular viability was determined by MTT test. Neurons cultured with URB597 (25, 50 or 100 nM) displayed a decrease in cellular viability. In addition, if cultured with OEA (25 nM) or PEA (100 nM), cellular death was found. These results further suggest that URB597, OEA or PEA promote cellular death.

AB - The endocannabinoid anandamide (ANA) participates in the control of cell death inducing the formation of apoptotic bodies and DNA fragmentation. The aim of this study was to evaluate whether the ANA degrading enzyme, the fatty acid amide hydrolase (FAAH), would induce cellular death. Experiments were performed in cerebellar granule neurons cultured with the FAAH inhibitor, URB597 (25, 50 or 100 nM) as well as endogenous lipids such as oleoylethanolamide (OEA) or palmitoylethanolamide (PEA) and cellular viability was determined by MTT test. Neurons cultured with URB597 (25, 50 or 100 nM) displayed a decrease in cellular viability. In addition, if cultured with OEA (25 nM) or PEA (100 nM), cellular death was found. These results further suggest that URB597, OEA or PEA promote cellular death.

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JF - International Archives of Medicine

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Cellular viability effects of fatty acid amide hydrolase inhibition on cerebellar neurons

Abstract

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