Circadian and sleep-deprivation variations of monophosphorylated MAP-Kinase in hypothalamus and pons of rats

Stephanie Mijangos-Moreno, Alwin Poot-Aké, Andrea Sarro-Ramírez, Ramsés Jiménez-Moreno, Elda Pacheco-Pantoja, Pedro Aquino-Hernández, Mireille Salas-Crisóstomo, Oscar Arias-Carrión, Eric Murillo-Rodríguez

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Behavior and physiological changes are under the influence of circadian and homeostatic variations. Temporal alignment regulates timing of neurobiological phenomena, such as protein phosphorylation. In the current report, we describe the circadian and sleep homeostatic phosphorylated mitogen-activated protein kinase (MAP-K) variations in hypothalamus and pons of rats across 24 h as well as after sleep deprivation. In the circadian study, MAP-K expression showed a building-up profile during the dark phase in hypothalamus, whereas an increase across the lights-on period was found in pons. On the other hand, that phosphorylation of MAP-K in hypothalamus and pons displayed a significant reduction after sleep rebound period. Data demonstrate that MAP-K phosphorylation undergoes circadian and sleep homeostatic variations in brain areas linked to sleep modulation.

Original languageEnglish
Pages (from-to)763-770
Number of pages8
JournalBiological Rhythm Research
Volume46
Issue number5
DOIs
StatePublished - 3 Sep 2015

Keywords

  • hypothalamus
  • mitogen-activated protein kinase
  • pons
  • sleep deprivation
  • Western blot

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