Constitutive and inducible nitric oxide synthase activities after spinal cord contusion in rats

Araceli Díaz-Ruiz; Antonio Ibarra; Francisca Pérez-Severiano; Gabriel Guízar-Sahagún; Israel Grijalva; Camilo Ríos

doi:10.1016/S0304-3940(01)02540-X

Constitutive and inducible nitric oxide synthase activities after spinal cord contusion in rats

Araceli Díaz-Ruiz, Antonio Ibarra, Francisca Pérez-Severiano, Gabriel Guízar-Sahagún, Israel Grijalva, Camilo Ríos

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

Nitric oxide (NO) plays a role in the secondary damage after spinal cord (SC) injury. NO is produced by the activity of two classes of enzymes: calcium-dependent constitutive nitric oxide synthase (NOS) and calcium-independent inducible NOS. To determine the time course of both NOS activities after SC injury, 50 Wistar rats were submitted to severe SC contusion. NOS activities were assayed at the site of SC injury at several times after lesion. Results showed a significant increase of 138 and 96% in the constitutive NOS activity at 4 and 8 h after the lesion, respectively, as compared to sham-operated rats. iNOS activity was increased 72 h after lesion by 103% (P < 0.05). In conclusion, both isoforms of NOS increase their activity at different time periods after SC injury.

Original language	English
Pages (from-to)	129-132
Number of pages	4
Journal	Neuroscience Letters
Volume	319
Issue number	3
DOIs	https://doi.org/10.1016/S0304-3940(01)02540-X
State	Published - 22 Feb 2002
Externally published	Yes

Keywords

Constitutive
Inducible
Nitric oxide
Spinal cord injury
Synthase

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10.1016/S0304-3940(01)02540-X

Cite this

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title = "Constitutive and inducible nitric oxide synthase activities after spinal cord contusion in rats",

abstract = "Nitric oxide (NO) plays a role in the secondary damage after spinal cord (SC) injury. NO is produced by the activity of two classes of enzymes: calcium-dependent constitutive nitric oxide synthase (NOS) and calcium-independent inducible NOS. To determine the time course of both NOS activities after SC injury, 50 Wistar rats were submitted to severe SC contusion. NOS activities were assayed at the site of SC injury at several times after lesion. Results showed a significant increase of 138 and 96% in the constitutive NOS activity at 4 and 8 h after the lesion, respectively, as compared to sham-operated rats. iNOS activity was increased 72 h after lesion by 103% (P < 0.05). In conclusion, both isoforms of NOS increase their activity at different time periods after SC injury.",

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T1 - Constitutive and inducible nitric oxide synthase activities after spinal cord contusion in rats

AU - Díaz-Ruiz, Araceli

AU - Ibarra, Antonio

AU - Pérez-Severiano, Francisca

AU - Guízar-Sahagún, Gabriel

AU - Grijalva, Israel

AU - Ríos, Camilo

PY - 2002/2/22

Y1 - 2002/2/22

N2 - Nitric oxide (NO) plays a role in the secondary damage after spinal cord (SC) injury. NO is produced by the activity of two classes of enzymes: calcium-dependent constitutive nitric oxide synthase (NOS) and calcium-independent inducible NOS. To determine the time course of both NOS activities after SC injury, 50 Wistar rats were submitted to severe SC contusion. NOS activities were assayed at the site of SC injury at several times after lesion. Results showed a significant increase of 138 and 96% in the constitutive NOS activity at 4 and 8 h after the lesion, respectively, as compared to sham-operated rats. iNOS activity was increased 72 h after lesion by 103% (P < 0.05). In conclusion, both isoforms of NOS increase their activity at different time periods after SC injury.

AB - Nitric oxide (NO) plays a role in the secondary damage after spinal cord (SC) injury. NO is produced by the activity of two classes of enzymes: calcium-dependent constitutive nitric oxide synthase (NOS) and calcium-independent inducible NOS. To determine the time course of both NOS activities after SC injury, 50 Wistar rats were submitted to severe SC contusion. NOS activities were assayed at the site of SC injury at several times after lesion. Results showed a significant increase of 138 and 96% in the constitutive NOS activity at 4 and 8 h after the lesion, respectively, as compared to sham-operated rats. iNOS activity was increased 72 h after lesion by 103% (P < 0.05). In conclusion, both isoforms of NOS increase their activity at different time periods after SC injury.

KW - Constitutive

KW - Inducible

KW - Nitric oxide

KW - Spinal cord injury

KW - Synthase

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Constitutive and inducible nitric oxide synthase activities after spinal cord contusion in rats

Abstract

Keywords

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