Cyclosporin-A inhibits inducible nitric oxide synthase activity and expression after spinal cord injury in rats

Araceli Diaz-Ruiz; Paula Vergara; Francisca Perez-Severiano; Jose Segovia; Gabriel Guizar-Sahagún; Antonio Ibarra; Camilo Ríos

doi:10.1016/j.neulet.2003.12.042

Cyclosporin-A inhibits inducible nitric oxide synthase activity and expression after spinal cord injury in rats

Araceli Diaz-Ruiz, Paula Vergara, Francisca Perez-Severiano, Jose Segovia, Gabriel Guizar-Sahagún, Antonio Ibarra, Camilo Ríos

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Nitric oxide is generated from L-arginine by a family of three distinct nitric oxide synthase (NOS) enzymes playing a crucial role in the physiopathology of spinal cord injury (SCI). Cyclosporin-A (CsA), an immunosupressive agent, may be used to inhibit the activity of iNOS and perhaps to protect against neural tissue destruction. Rats were submitted to SCI by contusion, and killed 4, 24 and 72 h after lesion. Results showed an increase in the activity of iNOS at 72 h after the SCI, inhibited by CsA (2.5 mg/kg) administered 12 h after trauma. iNOS Western blot assay showed an increase in the expression of iNOS after trauma, also antagonized by CsA administration.

Original language	English
Pages (from-to)	49-52
Number of pages	4
Journal	Neuroscience Letters
Volume	357
Issue number	1
DOIs	https://doi.org/10.1016/j.neulet.2003.12.042
State	Published - 26 Feb 2004
Externally published	Yes

Keywords

Cyclosporin-A
Neuroprotection
Nitric oxide synthase
Spinal cord injury

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10.1016/j.neulet.2003.12.042

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title = "Cyclosporin-A inhibits inducible nitric oxide synthase activity and expression after spinal cord injury in rats",

abstract = "Nitric oxide is generated from L-arginine by a family of three distinct nitric oxide synthase (NOS) enzymes playing a crucial role in the physiopathology of spinal cord injury (SCI). Cyclosporin-A (CsA), an immunosupressive agent, may be used to inhibit the activity of iNOS and perhaps to protect against neural tissue destruction. Rats were submitted to SCI by contusion, and killed 4, 24 and 72 h after lesion. Results showed an increase in the activity of iNOS at 72 h after the SCI, inhibited by CsA (2.5 mg/kg) administered 12 h after trauma. iNOS Western blot assay showed an increase in the expression of iNOS after trauma, also antagonized by CsA administration.",

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T1 - Cyclosporin-A inhibits inducible nitric oxide synthase activity and expression after spinal cord injury in rats

AU - Diaz-Ruiz, Araceli

AU - Vergara, Paula

AU - Perez-Severiano, Francisca

AU - Segovia, Jose

AU - Guizar-Sahagún, Gabriel

AU - Ibarra, Antonio

AU - Ríos, Camilo

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N2 - Nitric oxide is generated from L-arginine by a family of three distinct nitric oxide synthase (NOS) enzymes playing a crucial role in the physiopathology of spinal cord injury (SCI). Cyclosporin-A (CsA), an immunosupressive agent, may be used to inhibit the activity of iNOS and perhaps to protect against neural tissue destruction. Rats were submitted to SCI by contusion, and killed 4, 24 and 72 h after lesion. Results showed an increase in the activity of iNOS at 72 h after the SCI, inhibited by CsA (2.5 mg/kg) administered 12 h after trauma. iNOS Western blot assay showed an increase in the expression of iNOS after trauma, also antagonized by CsA administration.

AB - Nitric oxide is generated from L-arginine by a family of three distinct nitric oxide synthase (NOS) enzymes playing a crucial role in the physiopathology of spinal cord injury (SCI). Cyclosporin-A (CsA), an immunosupressive agent, may be used to inhibit the activity of iNOS and perhaps to protect against neural tissue destruction. Rats were submitted to SCI by contusion, and killed 4, 24 and 72 h after lesion. Results showed an increase in the activity of iNOS at 72 h after the SCI, inhibited by CsA (2.5 mg/kg) administered 12 h after trauma. iNOS Western blot assay showed an increase in the expression of iNOS after trauma, also antagonized by CsA administration.

KW - Cyclosporin-A

KW - Neuroprotection

KW - Nitric oxide synthase

KW - Spinal cord injury

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DO - 10.1016/j.neulet.2003.12.042

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Cyclosporin-A inhibits inducible nitric oxide synthase activity and expression after spinal cord injury in rats

Abstract

Keywords

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