TY - GEN
T1 - Diversity of Genotyping Chlamydia Trachomatis Serovars in Urogenital Samples from Mexican Patients
T2 - 45th Mexican Conference on Biomedical Engineering, CNIB 2022
AU - Hernández-Rosas, Fabiola
AU - García-González, Socorro Mariana
AU - Franco-González, Shumeyker Susmith
AU - Salgado-Álvarez, Ana Paola
AU - de León-Bautista, Mercedes Piedad
N1 - Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Nature Switzerland AG.
PY - 2023/1/1
Y1 - 2023/1/1
N2 - Chlamydia trachomatis (CT) is the most frequent bacterial sexually transmitted infection (STI) in the world. Therefore, the identification of serovars is essential for epidemiological surveillance and the development of prevention methods in our population. We aimed to demonstrate the diversity and frequency of serovars of CT in the urogenital tract in Mexican patients. We carried out an observational, prospective and cross-sectional study through 40 samples positive for CT. For the serovars identification, PCR multiplex, PCR of the ompA gene, automated sequencing, multiple sequence alignment, and phylogenetic analysis were used. Moreover, the variables like serovars, sex, aged groups, anatomical sites and, concomitant pathogens, were statistically analyzed. We reported that the prevalence of the most common serovars in our Mexican population were F (38.1%), E (27.3%) and, D (18.2%). Serovar F was the most prevalent in men, meanwhile, serovar D was in women and, E was equally prevalent in both genders. The most common concomitant pathogens were Ureaplasma spp. (22.7%), Mycoplasma spp. (13.6%) and, Haemophilus spp. (9.1%). In summary, epidemiological surveillance remarks the necessity of detecting serovars CT to elucidate the molecular implication and reduce sexual and reproductive complications.
AB - Chlamydia trachomatis (CT) is the most frequent bacterial sexually transmitted infection (STI) in the world. Therefore, the identification of serovars is essential for epidemiological surveillance and the development of prevention methods in our population. We aimed to demonstrate the diversity and frequency of serovars of CT in the urogenital tract in Mexican patients. We carried out an observational, prospective and cross-sectional study through 40 samples positive for CT. For the serovars identification, PCR multiplex, PCR of the ompA gene, automated sequencing, multiple sequence alignment, and phylogenetic analysis were used. Moreover, the variables like serovars, sex, aged groups, anatomical sites and, concomitant pathogens, were statistically analyzed. We reported that the prevalence of the most common serovars in our Mexican population were F (38.1%), E (27.3%) and, D (18.2%). Serovar F was the most prevalent in men, meanwhile, serovar D was in women and, E was equally prevalent in both genders. The most common concomitant pathogens were Ureaplasma spp. (22.7%), Mycoplasma spp. (13.6%) and, Haemophilus spp. (9.1%). In summary, epidemiological surveillance remarks the necessity of detecting serovars CT to elucidate the molecular implication and reduce sexual and reproductive complications.
KW - Automated sequencing
KW - Chlamydia trachomatis
KW - ompA gene
KW - PCR
KW - Serovar
UR - http://www.scopus.com/inward/record.url?scp=85142683571&partnerID=8YFLogxK
U2 - 10.1007/978-3-031-18256-3_13
DO - 10.1007/978-3-031-18256-3_13
M3 - Contribución a la conferencia
AN - SCOPUS:85142683571
SN - 9783031182556
T3 - IFMBE Proceedings
SP - 127
EP - 135
BT - 45th Mexican Conference on Biomedical Engineering - Proceedings of CNIB 2022
A2 - Trujillo-Romero, Citlalli Jessica
A2 - Gonzalez-Landaeta, Rafael
A2 - Chapa-González, Christian
A2 - Dorantes-Méndez, Guadalupe
A2 - Flores, Dora-Luz
A2 - Flores Cuautle, J.J.Agustin
A2 - Ortiz-Posadas, Martha R.
A2 - Salido Ruiz, Ricardo A.
A2 - Zuñiga-Aguilar, Esmeralda
PB - Springer Science and Business Media Deutschland GmbH
Y2 - 6 October 2022 through 8 October 2022
ER -