Exercise sensitizes skeletal muscle to extracellular ATP for IL-6 expression in mice

R. Fernández-Verdejo, M. Casas, J. E. Galgani, E. Jaimovich, S. Buvinic

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Active skeletal muscle synthesizes and releases interleukin-6 (IL-6), which plays important roles in the organism's adaptation to exercise. Autocrine/paracrine ATP signaling has been shown to modulate IL-6 expression. The aim of this study was to determine whether a period of physical activity modifies the ATP-induced IL-6 expression. BalbC mice were either subject to 5 weeks voluntary wheel running (VA) or kept sedentary (SED). Flexor digitorum brevis muscles were dissected, stimulated with different ATP concentrations (0-100 μM) and IL-6 mRNA levels were measured using qPCR. ATP evoked a concentration-dependent rise in IL-6 mRNA in both SED and VA mice. VA mice however, had significantly higher ATP sensitivity (pD2 pharmacological values: VA=5.58±0.02 vs. SED=4.95±0.04, p<0.05). Interestingly, in VA mice we observed a positive correlation between the level of physical activity and the IL-6 mRNA increase following fiber stimulation with 10μM ATP. In addition, there were lower P2Y2- and higher P2Y14-receptor mRNA levels in skeletal muscles of VA compared to SED mice, showing plasticity of nucleotide receptors with exercise. These results suggest that exercise increases skeletal muscle ATP sensitivity, a response dependent on the level of physical activity performed. This could have an important role in the mechanisms controlling skeletal muscle adaptation to exercise and training.

Original languageEnglish
Pages (from-to)273-279
Number of pages7
JournalInternational Journal of Sports Medicine
Volume35
Issue number4
DOIs
StatePublished - 1 Jan 2014
Externally publishedYes

Keywords

  • interleukin-6
  • nucleotide receptors
  • Physical activity
  • voluntary wheel running

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