TY - JOUR
T1 - Neuroprotection of completely lacerated spinal cord of adult rats by homotopic and heterotopic transplantation
AU - Guizar-Sahagun, Gabriel
AU - Grijalva, Israel
AU - Madrazo, Ignacio
AU - Franco-Bourland, Rebecca
AU - Salgado-Ceballos, Hermelinda
AU - Ibarra, Antonio
AU - Larriva-Sahd, Jorge
PY - 1994/1/1
Y1 - 1994/1/1
N2 - To evaluate the neuroprotective effect of transplants placed in the lesion zone after a complete spinal cord (SC) laceration, two independent series of experiments were carried out. In the first, allogeneic or xenogeneic fetal SC was transplanted into the gaps of the damaged lower thoracic SC of adult rats. In the transplanted rats the incidence of life-threatening complications was reduced, and the survival rate was increased compared with the control group (lesion, without implant). Histological examination showed less damage to the neighboring SC parenchyma in the transplanted rats. The measurement of this neuroprotective effect was made in a second series of experiments. Using the same model of SC injury, allogeneic fetal SC, autologous peripheral nerve and/or adipose tissue were implanted. Rats with implants of Gelfoam and damaged rats without implants were the controls. The implanted rats of all groups, including the Gelfoam group, showed a better survival rate than the nonimplanted rats. Significantly less damage to the neighboring SC parenchyma was measured in implanted rats with any of the live tissues tested compared with non-implanted rats, although no significant differences were observed between the Gelfoam group and the nonimplanted rats. Histological evidence of tissue implant survival was observed in all corresponding groups. It is concluded that the transplanted tissues tested here have a neuroprotective effect, possibly by acting as a buffer to neurotoxic substance(s) released by the stumps, and/or by exerting trophic effect(s) on the host.
AB - To evaluate the neuroprotective effect of transplants placed in the lesion zone after a complete spinal cord (SC) laceration, two independent series of experiments were carried out. In the first, allogeneic or xenogeneic fetal SC was transplanted into the gaps of the damaged lower thoracic SC of adult rats. In the transplanted rats the incidence of life-threatening complications was reduced, and the survival rate was increased compared with the control group (lesion, without implant). Histological examination showed less damage to the neighboring SC parenchyma in the transplanted rats. The measurement of this neuroprotective effect was made in a second series of experiments. Using the same model of SC injury, allogeneic fetal SC, autologous peripheral nerve and/or adipose tissue were implanted. Rats with implants of Gelfoam and damaged rats without implants were the controls. The implanted rats of all groups, including the Gelfoam group, showed a better survival rate than the nonimplanted rats. Significantly less damage to the neighboring SC parenchyma was measured in implanted rats with any of the live tissues tested compared with non-implanted rats, although no significant differences were observed between the Gelfoam group and the nonimplanted rats. Histological evidence of tissue implant survival was observed in all corresponding groups. It is concluded that the transplanted tissues tested here have a neuroprotective effect, possibly by acting as a buffer to neurotoxic substance(s) released by the stumps, and/or by exerting trophic effect(s) on the host.
KW - Autophagia
KW - Life-threatening complications
KW - Neural transplantation
KW - Neuroprotection
KW - Paraplegia
KW - Post-traumatic neurodegeneration
KW - Rat spinal cord injury
UR - http://www.scopus.com/inward/record.url?scp=0028113525&partnerID=8YFLogxK
U2 - 10.3233/RNN-1994-7201
DO - 10.3233/RNN-1994-7201
M3 - Artículo
AN - SCOPUS:0028113525
SN - 0922-6028
VL - 7
SP - 61
EP - 70
JO - Restorative Neurology and Neuroscience
JF - Restorative Neurology and Neuroscience
IS - 2
ER -