TY - JOUR
T1 - Potential therapeutic applications of mucuna pruriens peptide fractions purified by high-performance liquid chromatography as angiotensin-converting enzyme inhibitors, antioxidants, antithrombotic and hypocholesterolemic agents
AU - Herrera-Chalé, Francisco
AU - Ruiz-Ruiz, Jorge Carlos
AU - Betancur-Ancona, David
AU - Segura-Campos, Maira Rubi
N1 - Publisher Copyright:
© Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition 2016.
PY - 2016/2/1
Y1 - 2016/2/1
N2 - A Mucuna pruriens protein concentrate was hydrolyzed with a digestive (pepsin-pancreatin) enzymatic system. The soluble portion of the hydrolysate was fractionated by ultrafiltration and the ultrafiltered peptide fraction (PF) with lower molecular weight was purified by reversed-phase high-performance liquid chromatography. The PF obtained were evaluated by testing the biological activity in vitro. Fractions showed that the ability to inhibit the angiotensin-converting enzyme had IC50 values that ranged from 2.7 to 6.2 μg/mL. Trolox equivalent antioxidant capacity values ranged from 132.20 to 507.43 mM/mg. The inhibition of human platelet aggregation ranged from 1.59% to 11.11%, and the inhibition of cholesterol micellar solubility ranged from 0.24% to 0.47%. Hydrophobicity, size, and amino acid sequence could be factors in determining the biological activity of peptides contained in fractions. This is the first report that M. pruriens peptides act as antihypertensives, antioxidants, and inhibitors for human platelet aggregation and cholesterol micellar solubility in vitro.
AB - A Mucuna pruriens protein concentrate was hydrolyzed with a digestive (pepsin-pancreatin) enzymatic system. The soluble portion of the hydrolysate was fractionated by ultrafiltration and the ultrafiltered peptide fraction (PF) with lower molecular weight was purified by reversed-phase high-performance liquid chromatography. The PF obtained were evaluated by testing the biological activity in vitro. Fractions showed that the ability to inhibit the angiotensin-converting enzyme had IC50 values that ranged from 2.7 to 6.2 μg/mL. Trolox equivalent antioxidant capacity values ranged from 132.20 to 507.43 mM/mg. The inhibition of human platelet aggregation ranged from 1.59% to 11.11%, and the inhibition of cholesterol micellar solubility ranged from 0.24% to 0.47%. Hydrophobicity, size, and amino acid sequence could be factors in determining the biological activity of peptides contained in fractions. This is the first report that M. pruriens peptides act as antihypertensives, antioxidants, and inhibitors for human platelet aggregation and cholesterol micellar solubility in vitro.
KW - biological activity
KW - Mucuna pruriens
KW - peptide fractions
KW - protein hydrolysate
KW - purification
UR - http://www.scopus.com/inward/record.url?scp=84958061723&partnerID=8YFLogxK
U2 - 10.1089/jmf.2015.0098
DO - 10.1089/jmf.2015.0098
M3 - Artículo
C2 - 26854846
AN - SCOPUS:84958061723
SN - 1096-620X
VL - 19
SP - 187
EP - 195
JO - Journal of Medicinal Food
JF - Journal of Medicinal Food
IS - 2
ER -