TY - JOUR
T1 - Prophylactic neuroprotection with A91 improves the outcome of spinal cord injured rats
AU - Ibarra, A.
AU - Sosa, M.
AU - García, E.
AU - Flores, A.
AU - Cruz, Y.
AU - Mestre, H.
AU - Martiñón, S.
AU - Pineda-Rodríguez, B. A.
AU - Gutiérrez-Ospina, G.
PY - 2013/10/25
Y1 - 2013/10/25
N2 - Iatrogenic injury to the spinal cord (SC) is not an uncommon complication of spinal surgery. In an attempt to establish a preventive therapy for anticipated SC injury, we tested the effect of a single dose (SD) vaccine vs. the addition of a booster dose (BD) of a neural-derived peptide (A91) prior to SC contusion. Immunization with A91 immediately after SC injury has demonstrated to induce significant tissue protection and motor recovery. After injury, only the BD vaccination schedule had a neuroprotective effect. It was capable of improving neurological recovery that was always significantly higher than the one observed in rats with SD immunization or those only treated with PBS. Toward the end of study, animals treated with an A91 BD presented a BBB score of 9.75. ±. 0.17 (mean. ±. standard deviation) while rats treated with SD or PBS had a score of 6.6. ±. 0.7 and 5.6. ±. 0.6 respectively. In the next step we attempted to corroborate the neuroprotective effect induced by A91 immunization. For this purpose, we assessed the survival of rubrospinal neurons (RSNs) and ventral horn neurons (VHNs) sixty days after SC injury. BD vaccination induced a significant survival of both RSNs and VHNs after injury. Finally, the failure or success of this therapy (SD or BD respectively) was associated with a lower (SD) or higher (BD) A91-specific T cell proliferation. Prophylactic neuroprotection with an initial and subsequent booster dose of A91 may improve recovery after SC injury sustained during invasive spinal surgery procedures.
AB - Iatrogenic injury to the spinal cord (SC) is not an uncommon complication of spinal surgery. In an attempt to establish a preventive therapy for anticipated SC injury, we tested the effect of a single dose (SD) vaccine vs. the addition of a booster dose (BD) of a neural-derived peptide (A91) prior to SC contusion. Immunization with A91 immediately after SC injury has demonstrated to induce significant tissue protection and motor recovery. After injury, only the BD vaccination schedule had a neuroprotective effect. It was capable of improving neurological recovery that was always significantly higher than the one observed in rats with SD immunization or those only treated with PBS. Toward the end of study, animals treated with an A91 BD presented a BBB score of 9.75. ±. 0.17 (mean. ±. standard deviation) while rats treated with SD or PBS had a score of 6.6. ±. 0.7 and 5.6. ±. 0.6 respectively. In the next step we attempted to corroborate the neuroprotective effect induced by A91 immunization. For this purpose, we assessed the survival of rubrospinal neurons (RSNs) and ventral horn neurons (VHNs) sixty days after SC injury. BD vaccination induced a significant survival of both RSNs and VHNs after injury. Finally, the failure or success of this therapy (SD or BD respectively) was associated with a lower (SD) or higher (BD) A91-specific T cell proliferation. Prophylactic neuroprotection with an initial and subsequent booster dose of A91 may improve recovery after SC injury sustained during invasive spinal surgery procedures.
KW - A91
KW - Neural constituents
KW - Paraplegia
KW - Prophylactic neuroprotection
KW - Protective autoimmunity
KW - Vaccination
UR - http://www.scopus.com/inward/record.url?scp=84884379398&partnerID=8YFLogxK
U2 - 10.1016/j.neulet.2013.08.048
DO - 10.1016/j.neulet.2013.08.048
M3 - Comentario/Debate
C2 - 24012811
AN - SCOPUS:84884379398
SN - 0304-3940
VL - 554
SP - 59
EP - 63
JO - Neuroscience Letters
JF - Neuroscience Letters
ER -