TY - JOUR
T1 - The administration of endocannabinoid uptake inhibitors OMDM-2 or VDM-11 promotes sleep and decreases extracellular levels of dopamine in rats
AU - Murillo-Rodríguez, Eric
AU - Palomero-Rivero, Marcela
AU - Millán-Aldaco, Diana
AU - Di Marzo, Vincenzo
PY - 2013/1/17
Y1 - 2013/1/17
N2 - The family of the endocannabinoid system comprises endogenous lipids (such as anandamide [ANA]), receptors (CB1/CB2 cannabinoid receptors), metabolic enzymes (fatty acid amide hydrolase [FAAH]) and a putative membrane transporter (anandamide membrane transporter [AMT]). Although the role of ANA, FAAH or the CB1 cannabinoid receptor in sleep modulation has been reported, the effects of the inhibition of AMT on sleep remain unclear. In the present study, we show that microdialysis perfusion in rats of AMT inhibitors, (9Z)-N-[1-((R)-4-hydroxbenzyl)-2-hydroxyethyl]-9-octadecenamide (OMDM-2) or N-(4-hydroxy-2-methylphenyl)-5Z,8Z,11Z,14Z-eicosatetraenamide (VDM-11; 10, 20 or 30μM; each compound) delivered into the paraventricular thalamic nucleus (PVA) increased sleep and decreased waking. In addition, the infusion of compounds reduced the extracellular levels of dopamine collected from nucleus accumbens. Taken together, these findings illustrate a critical role of AMT in sleep modulation.
AB - The family of the endocannabinoid system comprises endogenous lipids (such as anandamide [ANA]), receptors (CB1/CB2 cannabinoid receptors), metabolic enzymes (fatty acid amide hydrolase [FAAH]) and a putative membrane transporter (anandamide membrane transporter [AMT]). Although the role of ANA, FAAH or the CB1 cannabinoid receptor in sleep modulation has been reported, the effects of the inhibition of AMT on sleep remain unclear. In the present study, we show that microdialysis perfusion in rats of AMT inhibitors, (9Z)-N-[1-((R)-4-hydroxbenzyl)-2-hydroxyethyl]-9-octadecenamide (OMDM-2) or N-(4-hydroxy-2-methylphenyl)-5Z,8Z,11Z,14Z-eicosatetraenamide (VDM-11; 10, 20 or 30μM; each compound) delivered into the paraventricular thalamic nucleus (PVA) increased sleep and decreased waking. In addition, the infusion of compounds reduced the extracellular levels of dopamine collected from nucleus accumbens. Taken together, these findings illustrate a critical role of AMT in sleep modulation.
KW - Anandamide membrane transporter
KW - Dopamine
KW - Nucleus accumbens
KW - Rapid eye movement sleep
KW - Wakefulness
UR - http://www.scopus.com/inward/record.url?scp=84875922625&partnerID=8YFLogxK
U2 - 10.1016/j.physbeh.2012.11.007
DO - 10.1016/j.physbeh.2012.11.007
M3 - Artículo
AN - SCOPUS:84875922625
SN - 0031-9384
VL - 109
SP - 88
EP - 95
JO - Physiology and Behavior
JF - Physiology and Behavior
IS - 1
ER -