TY - JOUR
T1 - Autoreactivity against myelin basic protein in patients with chronic paraplegia
AU - Zajarías-Fainsod, D.
AU - Carrillo-Ruiz, J.
AU - Mestre, H.
AU - Grijalva, I.
AU - Madrazo, I.
AU - Ibarra, A.
PY - 2012/5/1
Y1 - 2012/5/1
N2 - Introduction Previous studies have shown the existence of either cellular or humoral MBP-reactive elements up to 5 years after spinal cord injury (SCI), but not the presence of both after 10 years. Materials and methods Twelve SCI patients, with more than 10 years of evolution, and 18 healthy blood donors were studied. Lymphocyte proliferation (colorimetric- BrdU ELISA assay) and antibody titers against MBP (ELISA Human IgG MBP-specific assay) were assessed. Results SCI patients presented a significant T-cell proliferation against MBP (lymphocyte proliferation index: 3.7 ± 1.5, mean ± SD) compared to control individuals (0.7 ± 0.3; P<0.001). Humoral response analysis yielded a significant difference (P<0.0001) between the antibody titers of controls and SCI patients. A significant correlation between cellular and humoral responses was observed. Finally, patients with an ASIA B presented the highest immune responses. Conclusion This work demonstrates, for the first time, the existence of both cellular and humoral responses against MBP in the chronic stages (>10 years) of injury.
AB - Introduction Previous studies have shown the existence of either cellular or humoral MBP-reactive elements up to 5 years after spinal cord injury (SCI), but not the presence of both after 10 years. Materials and methods Twelve SCI patients, with more than 10 years of evolution, and 18 healthy blood donors were studied. Lymphocyte proliferation (colorimetric- BrdU ELISA assay) and antibody titers against MBP (ELISA Human IgG MBP-specific assay) were assessed. Results SCI patients presented a significant T-cell proliferation against MBP (lymphocyte proliferation index: 3.7 ± 1.5, mean ± SD) compared to control individuals (0.7 ± 0.3; P<0.001). Humoral response analysis yielded a significant difference (P<0.0001) between the antibody titers of controls and SCI patients. A significant correlation between cellular and humoral responses was observed. Finally, patients with an ASIA B presented the highest immune responses. Conclusion This work demonstrates, for the first time, the existence of both cellular and humoral responses against MBP in the chronic stages (>10 years) of injury.
KW - Humoral response
KW - IgG
KW - Spinal cord injury
KW - T-cell response
UR - http://www.scopus.com/inward/record.url?scp=84862901123&partnerID=8YFLogxK
U2 - 10.1007/s00586-011-2060-7
DO - 10.1007/s00586-011-2060-7
M3 - Artículo
C2 - 22057439
AN - SCOPUS:84862901123
SN - 0940-6719
VL - 21
SP - 964
EP - 970
JO - European Spine Journal
JF - European Spine Journal
IS - 5
ER -