Exploring the Biological Potential of Hydroxyapatite-Doped with Magnesium: Cytotoxicity and Cell Viability Assessment

Rafael Rangel Ibarra, Juan David Olivares Hernández, José Rafael Alanis Gómez, Fabiola Hernández-Rosas

Risultato della ricercapeer review

Abstract

Hydroxyapatite (HAp) is a bioceramic material of great interest in the field of tissue engineering and bone regeneration due to its high biocompatibility and bioactivity in the human body. HAp has been doped with different elements including magnesium, strontium, silver, among others. Particularly, magnesium-doped hydroxyapatite (HAp-Mg) appears to have high potential in biomedical applications. Therefore, it is relevant to characterize its biological compatibility and cytotoxicity. In this study, an in vitro model of fibroblasts obtained from chicken embryos was used to evaluate the effect of HAp-Mg on cell viability. HAp doped with 2% magnesium was synthesized using the Microwave-Assisted Hydrothermal Method. Subsequently, X-ray diffraction analysis was performed to characterize the HAp-Mg samples. Morphological and microstructural characterization of HAp-Mg was carried out using scanning electron microscopy (SEM). To evaluate the effect of HAp-Mg on cell viability, primary cultures of fibroblasts from 10-day-old chicken embryos (ED10) were obtained. The cultures were treated with different concentrations of HAp-Mg (0.1–100 µg/ml) for 24 h. Cell viability and cytotoxicity were then evaluated using MTT and AlamarBlue assays. The results revealed that the tested concentrations of HAp-Mg did not present significant cytotoxic effects. These findings suggest that HAp-Mg has excellent biological compatibility and can be considered as a promising material for biomedical applications.

Lingua originaleEnglish
Titolo della pubblicazione ospite46th Mexican Conference on Biomedical Engineering - Proceedings of CNIB 2023 - Volume 2
Sottotitolo della pubblicazione ospiteBiomechanics, Rehabilitation and Clinical Engineering
EditorJosé de Jesús Agustín Flores Cuautle, Balam Benítez-Mata, Ricardo Antonio Salido-Ruiz, Hugo A. Vélez-Pérez, Gustavo Adolfo Alonso-Silverio, Guadalupe Dorantes-Méndez, Aldo Rodrigo Mejía-Rodríguez, Esmeralda Zúñiga-Aguilar, Edgar Del Hierro-Gutiérrez
EditoreSpringer Science and Business Media Deutschland GmbH
Pagine115-121
Numero di pagine7
ISBN (stampa)9783031469350
DOI
Stato di pubblicazionePublished - 1 gen 2024
Evento46th Mexican Conference on Biomedical Engineering, CNIB 2023 - Villahermosa Tabasco
Durata: 2 nov 20234 nov 2023

Serie di pubblicazioni

NomeIFMBE Proceedings
Volume97
ISSN (stampa)1680-0737
ISSN (elettronico)1433-9277

Conference

Conference46th Mexican Conference on Biomedical Engineering, CNIB 2023
Paese/TerritorioMexico
CittàVillahermosa Tabasco
Periodo2/11/234/11/23

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