TY - GEN
T1 - Exploring the Biological Potential of Hydroxyapatite-Doped with Magnesium
T2 - 46th Mexican Conference on Biomedical Engineering, CNIB 2023
AU - Ibarra, Rafael Rangel
AU - Hernández, Juan David Olivares
AU - Gómez, José Rafael Alanis
AU - Hernández-Rosas, Fabiola
N1 - Publisher Copyright:
© The Author(s), under exclusive license to Springer Nature Switzerland AG 2024.
PY - 2024/1/1
Y1 - 2024/1/1
N2 - Hydroxyapatite (HAp) is a bioceramic material of great interest in the field of tissue engineering and bone regeneration due to its high biocompatibility and bioactivity in the human body. HAp has been doped with different elements including magnesium, strontium, silver, among others. Particularly, magnesium-doped hydroxyapatite (HAp-Mg) appears to have high potential in biomedical applications. Therefore, it is relevant to characterize its biological compatibility and cytotoxicity. In this study, an in vitro model of fibroblasts obtained from chicken embryos was used to evaluate the effect of HAp-Mg on cell viability. HAp doped with 2% magnesium was synthesized using the Microwave-Assisted Hydrothermal Method. Subsequently, X-ray diffraction analysis was performed to characterize the HAp-Mg samples. Morphological and microstructural characterization of HAp-Mg was carried out using scanning electron microscopy (SEM). To evaluate the effect of HAp-Mg on cell viability, primary cultures of fibroblasts from 10-day-old chicken embryos (ED10) were obtained. The cultures were treated with different concentrations of HAp-Mg (0.1–100 µg/ml) for 24 h. Cell viability and cytotoxicity were then evaluated using MTT and AlamarBlue assays. The results revealed that the tested concentrations of HAp-Mg did not present significant cytotoxic effects. These findings suggest that HAp-Mg has excellent biological compatibility and can be considered as a promising material for biomedical applications.
AB - Hydroxyapatite (HAp) is a bioceramic material of great interest in the field of tissue engineering and bone regeneration due to its high biocompatibility and bioactivity in the human body. HAp has been doped with different elements including magnesium, strontium, silver, among others. Particularly, magnesium-doped hydroxyapatite (HAp-Mg) appears to have high potential in biomedical applications. Therefore, it is relevant to characterize its biological compatibility and cytotoxicity. In this study, an in vitro model of fibroblasts obtained from chicken embryos was used to evaluate the effect of HAp-Mg on cell viability. HAp doped with 2% magnesium was synthesized using the Microwave-Assisted Hydrothermal Method. Subsequently, X-ray diffraction analysis was performed to characterize the HAp-Mg samples. Morphological and microstructural characterization of HAp-Mg was carried out using scanning electron microscopy (SEM). To evaluate the effect of HAp-Mg on cell viability, primary cultures of fibroblasts from 10-day-old chicken embryos (ED10) were obtained. The cultures were treated with different concentrations of HAp-Mg (0.1–100 µg/ml) for 24 h. Cell viability and cytotoxicity were then evaluated using MTT and AlamarBlue assays. The results revealed that the tested concentrations of HAp-Mg did not present significant cytotoxic effects. These findings suggest that HAp-Mg has excellent biological compatibility and can be considered as a promising material for biomedical applications.
KW - Biological Assay
KW - Biomaterials
KW - Hydroxyapatite
KW - Tissue Engineering
UR - http://www.scopus.com/inward/record.url?scp=85177480266&partnerID=8YFLogxK
U2 - 10.1007/978-3-031-46936-7_12
DO - 10.1007/978-3-031-46936-7_12
M3 - Contribución a la conferencia
AN - SCOPUS:85177480266
SN - 9783031469350
T3 - IFMBE Proceedings
SP - 115
EP - 121
BT - 46th Mexican Conference on Biomedical Engineering - Proceedings of CNIB 2023 - Volume 2
A2 - Flores Cuautle, José de Jesús Agustín
A2 - Benítez-Mata, Balam
A2 - Salido-Ruiz, Ricardo Antonio
A2 - Vélez-Pérez, Hugo A.
A2 - Alonso-Silverio, Gustavo Adolfo
A2 - Dorantes-Méndez, Guadalupe
A2 - Mejía-Rodríguez, Aldo Rodrigo
A2 - Zúñiga-Aguilar, Esmeralda
A2 - Hierro-Gutiérrez, Edgar Del
PB - Springer Science and Business Media Deutschland GmbH
Y2 - 2 November 2023 through 4 November 2023
ER -