TY - JOUR
T1 - Pigment epithelium derived factor (PEDF) expression in the male tract of Wistar rats
AU - Conte, Maria Ines
AU - Cabrillana, Maria Eugenia
AU - Saez Lancellotti, Tania Estefania
AU - Simon, Layla
AU - Funes, Abi Karenina
AU - Cayado-Gutiérrez, Niubys
AU - Tagle-Delgado, Matias Gustavo
AU - Vincenti, Amanda Edith
AU - Lopez, Maria Elis
AU - Pietrobon, Elisa Olivia
AU - Fornes, Miguel Walter
AU - Monclus, Maria Angeles
N1 - Publisher Copyright:
© 2018 Elsevier Inc.
PY - 2018/9/26
Y1 - 2018/9/26
N2 - Pigment epithelium derived factor (PEDF) expression has been described in many organs as showing neurotrophic, anti-angiogenic, anti-apoptotic, anti-inflammatory, anti-oxidant and pro-cell survival properties. However, references to its activity in the male reproductive system are scarce. We aimed to characterize the expression of PEDF in the male reproductive tract of Wistar rats by using RT-PCR, western blot and immunostaining and also evaluate the effect of flutamide in PEDF expression. We found that PEDF is expressed in the epididymis, prostate and seminal vesicles in Wistar rats, but notably not in the testes. Under the effect of flutamide PEDF expression decreased, recovering by suppressing the antiandrogen. The epididymis is an essential organ in sperm maturation-storages. The role of PEDF in this physiological process has not been fully elucidated yet, but considering that in other systems PEDF has anti-apoptotic, anti-oxidants and pro-cell survival properties, its expression along the epididymis could play a role in the protection of spermatozoa while they are stored.
AB - Pigment epithelium derived factor (PEDF) expression has been described in many organs as showing neurotrophic, anti-angiogenic, anti-apoptotic, anti-inflammatory, anti-oxidant and pro-cell survival properties. However, references to its activity in the male reproductive system are scarce. We aimed to characterize the expression of PEDF in the male reproductive tract of Wistar rats by using RT-PCR, western blot and immunostaining and also evaluate the effect of flutamide in PEDF expression. We found that PEDF is expressed in the epididymis, prostate and seminal vesicles in Wistar rats, but notably not in the testes. Under the effect of flutamide PEDF expression decreased, recovering by suppressing the antiandrogen. The epididymis is an essential organ in sperm maturation-storages. The role of PEDF in this physiological process has not been fully elucidated yet, but considering that in other systems PEDF has anti-apoptotic, anti-oxidants and pro-cell survival properties, its expression along the epididymis could play a role in the protection of spermatozoa while they are stored.
KW - Epididymis
KW - Flutamide
KW - PEDF
KW - Sperm
UR - http://www.scopus.com/inward/record.url?scp=85052743902&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2018.08.165
DO - 10.1016/j.bbrc.2018.08.165
M3 - Artículo
C2 - 30180954
AN - SCOPUS:85052743902
SN - 0006-291X
VL - 504
SP - 257
EP - 262
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -